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- Aug 30, 2002
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A.robustusOriginally posted by Venom
Out of curiousity, do you have LD50 stats on Atrax/ Hadronyche, or know where they can be found?
It is a protein, MW 4854 (calculated from amino acid
sequence), with 42 amino acid residues, with a high
proportion of basic residues, 8 cysteine residues with 4
disulphide bridges and has a pI >9 and an LD50 (SC newborn
mice) of 0.16 mg/kg. Robustoxin alone causes a typical
syndrome of envenomation in primates and man of whole male
Atrax robustus venom. (Sheumack et al 1983, 1984, 1985)
Versutoxin (from male and female Hadronyche versuta venom.)
Structurally very close to robustoxin, a protein neurotoxin,
MW 4852, 42 residues, highly basic, pI >9, 4 disulphide
bridges. 8 residues different to robustoxin, and considered
conservative changes. LD50 (SC newborn mice) 0.22 mg/kg.
Versutoxin alone causes almost typical syndrome of
envenomation in primates and man of whole male Atrax
robustus venom, the difference being the sustained
hypotension typical of robustoxin, which is not seen with
either versutoxin or whole Hadronyche versuta venom. Strong
antigenic cross-reaction between robustoxin and versutoxin.
(Sheumack et al, 1984)
Venoms of important related species
Hadronyche formidabilis - Venom reported as approximately
equal in toxicity (both male and female) as male Atrax
robustus venom, has caused similar cases of envenomation in
man (no definite fatalities), and therefore probably
contains a robustoxin-like component. (Sutherland 1983)
Hadronyche infensa - Venom reported as approximately equal
or greater in toxicity (females and males) as male Atrax
robustus venom, and therefore may contain a robustoxin-like
component. (Sutherland 1983)
Cheers,
Steve