The short answer is: when the spermathecae show signs of darkening.
I encourage you to search on this platform. Many of the questions you may have, have already been asked and answered. There are many threads about this topic, and I found them in under a minute:
My Aphonopelma seemani just molted into her adult colors and the molt has well developed spermatheca. How do I know when she is matured ebough to breed?
I believe I have a four year old female and an immature male Brachypelma smithi pair. How do you know when female tarantulas are mature? Are there any signs or do you have to wait for them to tap the ground looking for a mate?
How do you know when a female spider is mature enough to breed? I have a 4.5 inch avicularia metallica(im pretty sure is female.. if someone would confirm on the sexing id thread i would really appreciate it :) ) I know they are supposed to get larger but i read on the breeding reports that a...
arachnoboards.com
Success with breeding, and if you have any specific questions that you cannot find the answer to by searching old threads, you can always ask here
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