depends on what you have to examine the exuvium. if you have access to a microscope, you can find out what sex the t is at quite a small size. i have a magnifying lens that allows me to get a nice close up look, and i have been able to sex younger ts thanks to it..but not as small as you can figure out with a microscope. if you have nothing other than eyes and a camera..the larger it gets, the easier it is, and i'd say the spermathecae get easily visible to the eye at around 3"
think soap and alcohol solution (lens cleaner is my favorite) and dissecting needles and tweezers,
A stereo-microscope at 20X-40X works wonders,
I have sexed T's at 1-1.5 inches.
at this size though I make "guesstimates" since sometimes the Uterus externus and spermathecae might not be present at smaller sizes.
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