- Joined
- May 11, 2008
- Messages
- 1,332
Since my rig -- cantilevered off of the top of a tripod and pointing down at the hair specimen -- is rather unstable in such a configuration, the 4um step size was selected so as to be redundant. Diffraction theory at this magnification/aperture/NA predicts no focus banding with a step size of 8um. So I've still got, under normal applications, plenty of breathing room before I'll need those hacks.Time to HACK!
But I truly have no idea how the guys cope who stack at 100X!!
I've received comments on these photos that they're too dark. Hey, I was just excited to have been able to get 10X tarantula stacks at all!! lol To be honest they look pretty good to me, though I'd prefer some fill light on the foot shot. But it's true, lighting of living subjects at these powers is going to prove to be a challenge as I can't wrap the subject in a diffusion cocoon the way it's often done with dried specimens. I don't know, maybe I'm chasing down a blind alley expecting to shoot live subjects at these powers ...