At the lab we expire fruit fly vials 10 days after the first eclosion date. You don't want vials with mold in it, because the flies will transfer that mold all over the place.
Also parasitic mites. At my lab, we do immunology studies and there are so many kinds of mites found actually on the fruit flies. Besdies that, depending on your substrate, it could get yeasty/moldy, so after a certain point the larvae will be getting nothing out of it.
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