OMG maybe a first ever!!!

[dragontears]

Ryan, when I processed your male B. smithi, I saw what I would call live sperm preserved in the testes. This picture:

shows healthy sperm that were alive and squirmy just prior to the death and preservation of the male.

When I took Lori's male G. rosea after he died, I sectioned his palps and found sperm that were tightly coiled into small compact balls. I'm assuming this means that the sperm was not viable prior to death/preservation. I haven't gotten a picture of that slide yet...we've been really busy at work.

I would assume a wet mount and a high res microscope would show you whether the sperm was viable or not.

Just my completely inexperienced two cents.

 

[Talkenlate04]

So just based off of how they look when dead you can tell if they were alive?
Does alive mean moving or just alive?
That is cool!
I am more excited now to test this out. What magnification do I need to see sperm like that?

 

[desertdweller]

The Rape of Nature

Michael de Montaigne's (1533-1592) plea:

"Let us give Nature a chance; she knows her business better than we do"

Hmm..... 'nuf said.

 

[Talkenlate04]

Michael de Montaigne's (1533-1592) plea:

"Let us give Nature a chance; she knows her business better than we do"

Hmm..... 'nuf said.

If you believe that, go release your collection of T's outside, nature will take care of them.

 

[timekeeper]

That's pretty cool Ryan, you're a daredevil

 

[dragontears]

So just based off of how they look when dead you can tell if they were alive?
Does alive mean moving or just alive?
That is cool!
I am more excited now to test this out. What magnification do I need to see sperm like that?

Based off how they looked under the scope, I am assuming the sperm in your male was viable and the sperm of Lori's male was dead. There's really nothing to base this off of and no documentation to read up on, but it would make sense...your male was in his prime and the sperm was in the testes...Lori's male was over a year past mature and the sperm was in a pedipalp bulb. When I look at the slides, the tissue has been preserved, processed, embedded, sectioned, and stained...nothing is alive and moving anymore, but based on the positions of the sperm, I am willing to believe that my theory is correct. I'll try to get a picture at work soon of what I think to be dead sperm and I'll let you decide.

 

[cacoseraph]

Michael de Montaigne's (1533-1592) plea:

"Let us give Nature a chance; she knows her business better than we do"

Hmm..... 'nuf said.

ah yes... the cutting edge of 16th century science. very applicable to today

 

[fishwithoutabik]

BTW, almost all livestock is conceived this way. They no longer take the oxen to the cows, it's all done with srynges and pipes and the animals can't doge the process either.

I am sure this will be a very unpopular opinion, but this whole artificial insemination of tarantulas issue really feels wrong to me.

 

[cacoseraph]

well... not knowing specifics of the two respective males


i would say the palpal sperm has the quiesence enforced on it and the sperm in the body is not yet put to sleep

i am almost positive i have read that the quiescent form is balled as lori's male's was described

 

[Veneficus]

...I have to admit man, I'm a little disturbed.

:?

I was thinking the exact same thing! lol.

 

[dragontears]

well... not knowing specifics of the two respective males


i would say the palpal sperm has the quiesence enforced on it and the sperm in the body is not yet put to sleep

i am almost positive i have read that the quiescent form is balled as lori's male's was described

Never mind then. I thought I was on to something there. Back to the drawing board. :wall:

 

[cacoseraph]

well... it woud probably be a good idea to confirm. i haven't read my tara books in over a year, iirc

 

[funnylori]

So I guess we just need to section the palps and testies of both a healthy male and an unhealthy male and see if there is a difference. I have an Ornithoctonus sp. male on his way out the door. He didn't calm down enough after the show to put with the female, and now he's curling up... Poor guy. Got stressed to death.

 

[YouLosePayUp]

Why are you guys (Talken/Caco) considering posible mutilation of a valuable female? Would it not be easier, since you'll be watching the males day and night for sperm webs, to make the female produce dud sacs and grab it from her on the first day?

CCTV hooked to the computer would aid in this theory too.

 

[Talkenlate04]

Why are you guys (Talken/Caco) considering posible mutilation of a valuable female? Would it not be easier, since you'll be watching the males day and night for sperm webs, to make the female produce dud sacs and grab it from her on the first day?

CCTV hooked to the computer would aid in this theory too.

Catching a female making a dud sac? Your kidding right? I think I have had maybe 2-3 dud sacs laid by females that were not bred in many many years, so I don't really think that would be a workable avenue.

 

[Tescos]

Catching a female making a dud sac? Your kidding right? I think I have had maybe 2-3 dud sacs laid by females that were not bred in many many years, so I don't really think that would be a workable avenue.

Well Mr science if you put that extra bit of thought into what you are thinking about doing this would be a much better option.
I personly think looking into ways how to store the sperm and eggs so that they remain fertile would be a better course to take, because what now happens if you go ahead and inject your female with the sperm and it goes on to produce a non fertile sac? You can draw no conclusions from it at all because you have absoultly no idea if if your aritficial insemination was a success as how are you going to see that it got to where it needed to be?

why not just try and start off small by just trying to fertilze the one egg?
then again totally ignor everything I have just written like is the normal for people such as yourself As I know hand on heart I am not worthie enough to even post on such a subject.

have fun
Chris

 

[Nich]

Isn't this in some way considered beastiality?

ROTFL!!!!!!!!!

Ryan, that's the craziest breeding situation of ever heard of.

 

[Talkenlate04]

Well Mr science if you put that extra bit of thought into what you are thinking about doing this would be a much better option.
I personly think looking into ways how to store the sperm and eggs so that they remain fertile would be a better course to take, because what now happens if you go ahead and inject your female with the sperm and it goes on to produce a non fertile sac? You can draw no conclusions from it at all because you have absoultly no idea if if your aritficial insemination was a success as how are you going to see that it got to where it needed to be?

why not just try and start off small by just trying to fertilze the one egg?
then again totally ignor everything I have just written like is the normal for people such as yourself As I know hand on heart I am not worthie enough to even post on such a subject.

have fun
Chris

Why can't you interject you opinion without being an ass? Are you capable of doing such a thing? Stay off my threads if you can't at least respond with the manners of a 10 year old.

I am not trying to be super scientific. I never will be. I am just doing things for fun. This whole conversation was mainly speaking of theory. Even if someone proves they can inseminate a female that would be to much trouble to go through to continue doing it. They do it best I will always agree with that.

 

[_bob_]

just a thought since this thread popped back up... since you were talking about using pipettes and what not.... what if you some how made a mold of the males bulb to use for the end of the pipette... ... if it was to actually work then you could have a pipette for each species... just a thought that ran across my head.

 

[Talkenlate04]

just a thought since this thread popped back up... since you were talking about using pipettes and what not.... what if you some how made a mold of the males bulb to use for the end of the pipette... ... if it was to actually work then you could have a pipette for each species... just a thought that ran across my head.

Hey that is a cool idea. That would be easy to do by using a dead MM palp for the mold.

I am just more curious if sperm will exist outside of the T and the palp.