Jumper necropsy results

Penguin

Arachnopeon
Joined
Jun 5, 2017
Messages
25
Hi everyone. I recently had necropsies performed on 4 of my jumpers. Let me explain a little before i get to that.
I raise captive bred regal jumping spiders, my facebook page dedicated to them can be veiwed here: https://www.facebook.com/Opheliathemonster/

About a year ago i started having an issue with many of them coming down with some sort of sickness. This was after i moved back to NJ from Florida where i began keeping them. Never had i had this issue with them while i was living there. But once i left, this ailment nearly wiped all my jumpers out twice!
They developed symptoms that could be lumped into the dks category, though they were a bit different then the videos i have seen of infected Ts. It started out with a twitch here and there but progressed over a few weeks. They stopped eating, drinking, defacating....thhey would start walking in jerky movements and eventually went into spasms. They would lay on their backs a lot, not sure if it was because they were too weak to turn over or if it was because that was the only way they could get relief from the spasms. The stopped moving their pedipalps and would keep clenching thier chelicarae. Very upsetting to watch. Occasionally i would be able to hand feed, or get them to drink water, but even the ones who showed improvement died. My first experience with this was last summer. I lost many adult regius, a few adult otiosis, and an entir clutch of regius slings. Then it stopped. After doing research o came to believe it was due to essential oils, as my family had them being diffused into the air compulsively, in like every room of the house. So we got rid of them. In the mean time i contacted exotic vets and etymologists but nobody had any info for me except dks.
But i thought the issue was gone as i went on to raise several clutches to adulthood. But then aboiut 2 months ago this same issue came around again. It was like the mysterious spider plague. Killed pff many of my adult regius, otiosis, my whole otiosis clutch, and.......dammit my hyllus diardi.
Interestingly enough, while it affected so many, there were many that remained fine. Which puzzled me.
So finally i ended up getting in touch with a vet who located a zoo pathologist who specializes in arachnids. They sent me a preservative to put my infected spiders in as soon as they passed.
I sent them 4 of them and they performed necropsies on them. Here is what i got back (this had to be cut and pasted btw)
Zoo/Exotic Pathology Service
6020 Rutland Drive #14
Carmichael CA 95608-0515
www.zooexotic.com
Doctor: Johnson
Clinic: Avian & Exotic Animal Care
8711 Fidelity Boulevard
Raleigh, NC 27617
Client: -
Date: June 28, 2018
Access: V181439
Species: Jumping spiders
Breed: Spiders
Sex: Female
Name: -
Age: -
Type: Post mortem jar
CLINICAL INFORMATION
There have been increased deaths among spiders that exhibit some neurologic clinical signs.
Four spiders are submitted: Subadult female Phidippus regius (Block A, blue thread); juvenile
female Hyllus diardi (Block B, yellow thread); adult female Phidippus regius (Block C, red
thread); and adult female regius (Block D, no thread, separate container).
MICROSCOPIC
Examined are sections of the spiders. The identified tissues may not be present in all sections
either due to autolysis or level of sectioning. These include skeletal muscle, particularly of the
limbs, heme lymph, exoskeleton, digestive organ, ovary (in varying stages of development),
digestive tube at different levels, book lungs, nerve bundles, hearts, eye, and Malpighian tubules.
Book lungs, Malpighian tubules, and neuro tissue are best identified on Block C.
Numerous bacteria, which are rod-shaped structures, can be identified on Block C and associated
with the degenerative gland and identified in the heme lymph. These are associated with areas of
significant cellular autolysis although the remainder of the tissues is well preserved. In Block D,
the digestive gland is supporting some variably-sized 3-4-µ round pale basophilic and rarely
eosinophilic cytoplasmic inclusions within the larger cells with basophilic cytoplasm. Similar
cytoplasmic inclusions could be identified in the well-preserved section of digestive gland in
Block B. I am interpreting this as normal. Coocobacillus bacteria are also identified within the
tissues of Block A, especially the digestive gland.
DIAGNOSIS
SPIDER BLOCKS A AND C: BACTERIAL PROLIFERATIONS
COMMENT
Multiple sections are examined of all four spiders. They are variably preserved with the
exception of some areas of the skeletal muscle (all spiders) and in ‘blue thread’ Block A spider
where there are large numbers of bacteria (cocci to coccobacillus) proliferating throughout the
digestive gland within the body. Bacteria, which are longer rod-shaped organisms, are identified
in the heme lymph and digestive organ of ‘no thread separate container’ Block C spider. I am
not seeing fungal organisms or metazoan parasites in the tissue sections of these spiders. The
exoskeleton appears intact in all of the spiders. Bacterial infections have been described in a
CONTINUED
V181439 Page 2
Number of arachnid as well as other insect species such as stick insects. The exact identity of
these bacteria is not determined. Culture may be helpful in identifying a bacterium in other
affected spiders. I suggest collection of the heme lymph from distal leg joints to prevent
contamination from the intestinal flora. In references some bacteria that have been identified in
spiders include Bacillus species; however, it is suspected that these may be related to husbandry
issues such as changes in humidity.
DRURY R. REAVILL, DVM
DABVP (Avian and Reptile & Amphibian Practice)
Diplomate, American College of Veterinary Pathologists
DRR:br* Q2 KW infection (bacteria)

So it appears they werw suffering from some sort of bacterial contamination. For them to find out thr exact strain will require more research. They also said there may be a way to treat it but to do this i would have to get the spider to drink the medicine, (if i can even afford such a thing) but by the time they show any definite signs of illness they refuse food and water....
If anyone can get anything out of this report and offer any suggestions as to either treatment or prevention, or any ideas on the cause.... i would greatly appreciate it. I have made a few adjustments as far as how i feed and store my feeders, and removed certain objects from their enclosures, and got springtails to put in with them, but is there anything else i should or should not be doing?
 

basin79

ArachnoGod
Active Member
Joined
Sep 14, 2013
Messages
5,893
Hi everyone. I recently had necropsies performed on 4 of my jumpers. Let me explain a little before i get to that.
I raise captive bred regal jumping spiders, my facebook page dedicated to them can be veiwed here: https://www.facebook.com/Opheliathemonster/

About a year ago i started having an issue with many of them coming down with some sort of sickness. This was after i moved back to NJ from Florida where i began keeping them. Never had i had this issue with them while i was living there. But once i left, this ailment nearly wiped all my jumpers out twice!
They developed symptoms that could be lumped into the dks category, though they were a bit different then the videos i have seen of infected Ts. It started out with a twitch here and there but progressed over a few weeks. They stopped eating, drinking, defacating....thhey would start walking in jerky movements and eventually went into spasms. They would lay on their backs a lot, not sure if it was because they were too weak to turn over or if it was because that was the only way they could get relief from the spasms. The stopped moving their pedipalps and would keep clenching thier chelicarae. Very upsetting to watch. Occasionally i would be able to hand feed, or get them to drink water, but even the ones who showed improvement died. My first experience with this was last summer. I lost many adult regius, a few adult otiosis, and an entir clutch of regius slings. Then it stopped. After doing research o came to believe it was due to essential oils, as my family had them being diffused into the air compulsively, in like every room of the house. So we got rid of them. In the mean time i contacted exotic vets and etymologists but nobody had any info for me except dks.
But i thought the issue was gone as i went on to raise several clutches to adulthood. But then aboiut 2 months ago this same issue came around again. It was like the mysterious spider plague. Killed pff many of my adult regius, otiosis, my whole otiosis clutch, and.......dammit my hyllus diardi.
Interestingly enough, while it affected so many, there were many that remained fine. Which puzzled me.
So finally i ended up getting in touch with a vet who located a zoo pathologist who specializes in arachnids. They sent me a preservative to put my infected spiders in as soon as they passed.
I sent them 4 of them and they performed necropsies on them. Here is what i got back (this had to be cut and pasted btw)
Zoo/Exotic Pathology Service
6020 Rutland Drive #14
Carmichael CA 95608-0515
www.zooexotic.com
Doctor: Johnson
Clinic: Avian & Exotic Animal Care
8711 Fidelity Boulevard
Raleigh, NC 27617
Client: -
Date: June 28, 2018
Access: V181439
Species: Jumping spiders
Breed: Spiders
Sex: Female
Name: -
Age: -
Type: Post mortem jar
CLINICAL INFORMATION
There have been increased deaths among spiders that exhibit some neurologic clinical signs.
Four spiders are submitted: Subadult female Phidippus regius (Block A, blue thread); juvenile
female Hyllus diardi (Block B, yellow thread); adult female Phidippus regius (Block C, red
thread); and adult female regius (Block D, no thread, separate container).
MICROSCOPIC
Examined are sections of the spiders. The identified tissues may not be present in all sections
either due to autolysis or level of sectioning. These include skeletal muscle, particularly of the
limbs, heme lymph, exoskeleton, digestive organ, ovary (in varying stages of development),
digestive tube at different levels, book lungs, nerve bundles, hearts, eye, and Malpighian tubules.
Book lungs, Malpighian tubules, and neuro tissue are best identified on Block C.
Numerous bacteria, which are rod-shaped structures, can be identified on Block C and associated
with the degenerative gland and identified in the heme lymph. These are associated with areas of
significant cellular autolysis although the remainder of the tissues is well preserved. In Block D,
the digestive gland is supporting some variably-sized 3-4-µ round pale basophilic and rarely
eosinophilic cytoplasmic inclusions within the larger cells with basophilic cytoplasm. Similar
cytoplasmic inclusions could be identified in the well-preserved section of digestive gland in
Block B. I am interpreting this as normal. Coocobacillus bacteria are also identified within the
tissues of Block A, especially the digestive gland.
DIAGNOSIS
SPIDER BLOCKS A AND C: BACTERIAL PROLIFERATIONS
COMMENT
Multiple sections are examined of all four spiders. They are variably preserved with the
exception of some areas of the skeletal muscle (all spiders) and in ‘blue thread’ Block A spider
where there are large numbers of bacteria (cocci to coccobacillus) proliferating throughout the
digestive gland within the body. Bacteria, which are longer rod-shaped organisms, are identified
in the heme lymph and digestive organ of ‘no thread separate container’ Block C spider. I am
not seeing fungal organisms or metazoan parasites in the tissue sections of these spiders. The
exoskeleton appears intact in all of the spiders. Bacterial infections have been described in a
CONTINUED
V181439 Page 2
Number of arachnid as well as other insect species such as stick insects. The exact identity of
these bacteria is not determined. Culture may be helpful in identifying a bacterium in other
affected spiders. I suggest collection of the heme lymph from distal leg joints to prevent
contamination from the intestinal flora. In references some bacteria that have been identified in
spiders include Bacillus species; however, it is suspected that these may be related to husbandry
issues such as changes in humidity.
DRURY R. REAVILL, DVM
DABVP (Avian and Reptile & Amphibian Practice)
Diplomate, American College of Veterinary Pathologists
DRR:br* Q2 KW infection (bacteria)

So it appears they werw suffering from some sort of bacterial contamination. For them to find out thr exact strain will require more research. They also said there may be a way to treat it but to do this i would have to get the spider to drink the medicine, (if i can even afford such a thing) but by the time they show any definite signs of illness they refuse food and water....
If anyone can get anything out of this report and offer any suggestions as to either treatment or prevention, or any ideas on the cause.... i would greatly appreciate it. I have made a few adjustments as far as how i feed and store my feeders, and removed certain objects from their enclosures, and got springtails to put in with them, but is there anything else i should or should not be doing?
Would you mind looking at a couple of videos of mine if I post them on here?

I lost 2 w/c Hyllus diardi and they had the same symptoms. I blamed the spot on flea treatment I used on my puppy outside.

I'm not looking to shift the blame from myself but it would be interesting to find out if mine acted like yours did.
 

boina

Lady of the mites
Active Member
Joined
Mar 25, 2015
Messages
2,217
Thank you for posting this, this is extremely interesting. There has been at least one case where a similar outbreak of neurological symptoms in a captive tarantula population has been caused by a bacterial infection.

@basin79 neurological symptoms may vary depending on the infecting bacterium, so even if your diardi didn't have exactly the same symptoms it may still have been a bacterial infection.
 

The wolf

Arachnolord
Joined
May 6, 2017
Messages
600
My hyllus diardi never ate or made a sac,began to slow down,never left the ground and eventually died,probably an age thing as she was wc

Sad really
 

Penguin

Arachnopeon
Joined
Jun 5, 2017
Messages
25
Would you mind looking at a couple of videos of mine if I post them on here?

I lost 2 w/c Hyllus diardi and they had the same symptoms. I blamed the spot on flea treatment I used on my puppy outside.

I'm not looking to shift the blame from myself but it would be interesting to find out if mine acted like yours did.
Yeah i can look at it. That was one of my suspicions as well for awhile but them i switched over to nexgard and it still happened. After contacting the company they were pretty convinced that unless the dog is actually bitten and blood is ingested that it is safe, cant aay they same for the frontline though as that is topical.
 

basin79

ArachnoGod
Active Member
Joined
Sep 14, 2013
Messages
5,893
Yeah i can look at it. That was one of my suspicions as well for awhile but them i switched over to nexgard and it still happened. After contacting the company they were pretty convinced that unless the dog is actually bitten and blood is ingested that it is safe, cant aay they same for the frontline though as that is topical.
Thank you.



 

Penguin

Arachnopeon
Joined
Jun 5, 2017
Messages
25
Wow that is very upsetting to watch. Mine had some similarities, yes. It almost looks like theyve been poisoned by something right?
 

The Snark

Dumpster Fire of the Gods
Old Timer
Joined
Aug 8, 2005
Messages
11,048
So it appears they werw suffering from some sort of bacterial contamination.
No control mentioned. So we don't know how common or prevalent those bacteria are. Without a control the bacteria may be as common as e-coli in the human gut. Not sure where the investigation can go from here.
 

basin79

ArachnoGod
Active Member
Joined
Sep 14, 2013
Messages
5,893
Wow that is very upsetting to watch. Mine had some similarities, yes. It almost looks like theyve been poisoned by something right?
Aye. It was absolutely gutting to watch. I thought they had DKS so was hoping they may moult. If for a single second I knew they weren't going to make it I'd have done right by them.

I absolutely love jumping spiders. So much personality but after I lost my 2nd lass I made the decision to not buy any again as I was convinced it was my pup's flea treatment. Still won't risk it as I still don't know the reason they died.

Appreciate you taking the time to watch the videos. Who you mind if I shared this thread in those videos and/or in a new video?
 

boina

Lady of the mites
Active Member
Joined
Mar 25, 2015
Messages
2,217
No control mentioned. So we don't know how common or prevalent those bacteria are. Without a control the bacteria may be as common as e-coli in the human gut. Not sure where the investigation can go from here.

which are rod-shaped structures, can be identified on Block C and associated
with the degenerative gland and identified in the heme lymph. These are associated with areas of
significant cellular autolysis although the remainder of the tissues is well preserved.
No, this description does not suggest commensal bacteria of the gut - at all. This really points to a bacterial infection. As far as it has been researched (which is not very far, I admit that) spiders don't have a regular bacterial population in their gut.
 

The Snark

Dumpster Fire of the Gods
Old Timer
Joined
Aug 8, 2005
Messages
11,048
As far as it has been researched (which is not very far, I admit that) spiders don't have a regular bacterial population in their gut.
I missed the autolysis. But populations, or lack thereof, still need to be determined.

@boina Also, a common cause needs to be determined, or a susceptibility to certain specific pathogens available in the environment. Any epidemiologists in the house?
 

Penguin

Arachnopeon
Joined
Jun 5, 2017
Messages
25
Aye. It was absolutely gutting to watch. I thought they had DKS so was hoping they may moult. If for a single second I knew they weren't going to make it I'd have done right by them.

I absolutely love jumping spiders. So much personality but after I lost my 2nd lass I made the decision to not buy any again as I was convinced it was my pup's flea treatment. Still won't risk it as I still don't know the reason they died.

Appreciate you taking the time to watch the videos. Who you mind if I shared this thread in those videos and/or in a new video?

Absolutely, please share! Anyone that this can help.
 

Razzledazzy

Arachnosquire
Joined
Mar 31, 2018
Messages
82
This is fascinating. It was very good of you to get in touch with a professional. Maybe we can cobble together some kind of treatment to prevent this from happening. You said it didn't happen in Florida? That's odd to me because you'd think bacteria would find the climate here more hospitable.
 

schmiggle

Arachnoking
Joined
Nov 3, 2013
Messages
2,220
You said it didn't happen in Florida? That's odd to me because you'd think bacteria would find the climate here more hospitable.
There could be any number of reasons it didn't happen in Florida. For example, if these attack spiders that are already weakened, then if the spiders prefer the climate in Florida they may never get weak enough to be attacked.
 
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